Journal: International Journal of Molecular Sciences

Article Title: Acetylated α-Tubulin and α-Synuclein: Physiological Interplay and Contribution to α-Synuclein Oligomerization

doi: 10.3390/ijms241512287

Figure Lengend Snippet: The increase of tubulin acetylation induced α-synuclein oligomers’ formation. ( A ) Acetylated α-tubulin (in green) and oligomeric α-synuclein (AS-PLA, in red) in SK-N-SH Syn + treated for 2 h with 12 µM Tubacin compared to DMSO control. The lower panels show the 2× magnified view of selected squared areas. Arrow: AS-PLA staining indicating oligomeric α-synuclein. Scale bar: 20 µm. ( B ) Graph represents the mean area of the oligomeric α-synuclein in the analyzed cells (expressed as area AS PLA/cell). N = 4 biological replicates, ** p = 0.0080, according to the paired t -test.

Article Snippet: After 6 days of differentiation, cells were treated with DMSO (vehicle, Euroclone) and 6 μM or 12 μM Tubacin (Sigma-Aldrich) for 2 h at 37 °C, gently washed twice with Phosphate-Buffered Saline (PBS, Biosigma, Cona, Italy), and processed for analyses.

Techniques: Control, Staining

Journal: Oncotarget

Article Title: TAK1 and IKK2, novel mediators of SCF-induced signaling and potential targets for c-Kit-driven diseases

doi:

Figure Lengend Snippet: Wt A–G. , myd88 −/− (A), lyn −/− (E), ikk2 F/F , ikk2 Δ (F), or DMSO (vehicle) or inhibitor treated [5 μM U-73122 (B), 5 μM SU6656 (C), 0,5 μM 5Z-7-Oxozeanol (5Z-7-Oxo) (D), 50 μM S3I-201 (G)] BMMCs were stimulated with SCF (50 ng/ml) alone or in combination with IL-33 (50 ng/ml). Cells were probed with [H 3 ]-thymidine and analyzed by β -counting (A–G). H, I. BMMCs were stimulated with SCF or IL-33 (both 50 ng/ml) or co-stimulated with both. Co-stimulation was performed by pre-incubation of the cells with SCF for 30min (pre-SCF 30min) followed by stimulation with IL-33 (for 10, 30, 60, 90 min). Lysates were analyzed by western blotting. J, K. HMC-1.1 cells were treated with the TAK1 inhibitor 5Z-7-oxozeanol (5Z-7-Ox.) (J) or the IKK-inhibitor VII (IKK-I VII) (K), and were stimulated with IL-33. Lysates were analyzed by western blotting.

Article Snippet: HMC-1.1 and HMC-1.2 cells (10 6 cells/ml) were treated with vehicle (DMSO) or inhibitors (60 min) (Merck Millipore).

Techniques: Incubation, Western Blot